Transcription factors (TFs) regulate gene expression by binding to cis-regulatory elements near target genes. They are universally important in all biological processes, but their study is hampered by the lack of molecular and genetic tools, which are only available for a subset of TFs. To overcome this issue, we will generate a library of fly lines with endogenously tagged TFs. Using our novel Exchangeable Tagging System (ExTaSy), we will add a swappable protein tag to every terminus of every annotated Drosophila TF gene.

We are looking for three new colleagues to join us at the Centre for Functional Genomics at Oxford Brookes University to help with the establishment of this library. We are a small group of enthusiastic researchers studying different aspects of gene regulation mainly using Drosophila as a model. The Centre is embedded in the vibrant Oxford Brookes research community with links to other scientists in Oxford, the UK, and abroad.

Postdoctoral Researcher (4 years full time, starting salary £33,314)
You will lead the lab work of the project, design transgenesis constructs, help with their molecular cloning, and help to generate transgenic fly strains using CRISPR knock-ins. You will also use immunostains on Drosophila tissues and confocal imaging to analyse transcription factor expression patterns as well as perform genome-wide transcription factor binding site analysis using CUT&RUN sequencing. The start date is flexible.
To apply for this position, please visit https://bit.ly/3eEwpMx.

Technical Research Associate (5 years full time, starting salary £28,762)
You will be responsible for the streamlined molecular cloning of the transgenesis constructs and the establishment of the transgenic fly strains through micro-injections and Drosophila genetics. The start date is as soon as possible.
To apply for this position, please visit https://bit.ly/3BbonCt.

Technical Research Assistant (5 years part time, starting salary £25,642)
You will help with the generation of the library and the maintenance of the fly strains by preparing fly food and providing administrative support to the research group. The start date is as soon as possible.
To apply for this position, please visit https://bit.ly/3Djerti.

All three staff will be based at the Centre for Functional Genomics at Oxford Brookes University (https://bit.ly/3uUrYSi) and collaborate closely with Korneel Hens (https://bit.ly/3eK99wS) and Sebastian Kittelmann (https://bit.ly/3ueqras), as well as Jack Sunter (https://bit.ly/3QMCN1S) from the Centre for Bioimaging (https://bit.ly/3dcNixB).

Please contact Korneel Hens (khens@brookes.ac.uk) or Sebastian Kittelmann (skittelmann@brookes.ac.uk) for informal inquiries.